The human microbiome, much like our fingerprint, is unique among individuals. Furthermore, there is significant intrapersonal variation among body sites. These complex microbial communities form distinct ecosystems across the body, some of which are of particular interest in the study of disease. I hypothesize that malodor forms at sites of prosthetic use in persons with amputations as a result of specific bacterial communities. This malodor may indicate a skin microbiome of aerotolerant organisms dominated by anaerobic bacteria. To this end, I expect Corynebacterium, Propionibacterium, Staphylococcus aureus, Staphylococcus epidermidis, and coagulase-negative Staphylococcus to be the most abundant species present, which contribute to the odor common among the residual limbs of prosthetic users, often leading to dermatitis. This study provides a non-invasive method for collecting host-associated bacterial skin samples from human volunteers. The purpose stands to characterize the microbial composition present at the site of socket wear, in order to understand the function of these communities of bacteria in dysbiosis and disease.