Researchers initially believed that the composition of the gut microbiome solely impacted physical health; however, recent discoveries regarding the gut-brain-microbiome connection have transformed the way psychobiologists conceptualize this system. Specifically, psychological stress and the microbiome are thought to have reciprocal links, thus we will explore whether these connections are present and how the parent-child bond may alter the nature of the stress-microbiome connection. Further, this project will analyze microbial composition in human parents and their children with the goal of discovering commonalities and differences in the microbiome within families. By analyzing the gut microbiota of 100 parent-child dyads, this project may inform understanding of the development of the personalized microbiome as it corresponds to age, health status, social relationships, and psychological stress. Thus, this work will cross a new frontier at the interface of multiple disciplines–exploring the multifaceted connections between psychological health and microbial composition across generations.